|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37648||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerChang-Zheng Chen Lab
- Backbone size (bp) 7066
Vector typeMammalian Expression, Retroviral
- Promoter mPGK
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
- Cloning method Restriction Enzyme
- 5′ sequencing primer mPGK-F
- 3′ sequencing primer IRES-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MXW-PGK-IRES-EGFP was a gift from Chang-Zheng Chen (Addgene plasmid # 37648 ; http://n2t.net/addgene:37648 ; RRID:Addgene_37648)
For your References section:Loop nucleotides control primary and mature miRNA function in target recognition and repression. Yue SB, Trujillo RD, Tang Y, O'Gorman WE, Chen CZ. RNA Biol. 2011 Nov-Dec;8(6):1115-23. 10.4161/rna.8.6.17626 PubMed 22142974