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pASCV-HhN-Ren
(Plasmid #37676)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37676 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HhN
  • Species
    D. melanogaster (fly)
  • Promoter Actin 5C
  • Tag / Fusion Protein
    • renilla luciferase (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer AC5
  • 3′ sequencing primer BGH-Rev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Renilla inserted at aa254 of HhN

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pASCV-HhN-Ren was a gift from Philip Beachy (Addgene plasmid # 37676 ; http://n2t.net/addgene:37676 ; RRID:Addgene_37676)
  • For your References section:

    The ihog cell-surface proteins bind Hedgehog and mediate pathway activation. Yao S, Lum L, Beachy P. Cell. 2006 Apr 21;125(2):343-57. 10.1016/j.cell.2006.02.040 PubMed 16630821