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PIGAZFN-R2
(Plasmid #37800)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 37800 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pST1374
  • Backbone manufacturer
    Keith Joung lab @ MGH/HMS
  • Backbone size w/o insert (bp) 5709
  • Total vector size (bp) 5973
  • Modifications to backbone
    The backbone was digested using XbaI/BamHI to insert the zinc finger sequence.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PIGA zinc finger R2
  • Species
    Synthetic
  • Insert Size (bp)
    264
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The insert and backbone were from Dr. Keith Joung's lab in Massachusetts General Hospital/Harvard Medical School.
  • Terms and Licenses

Depositor Comments

Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PIGAZFN-R2 was a gift from Linzhao Cheng (Addgene plasmid # 37800 ; http://n2t.net/addgene:37800 ; RRID:Addgene_37800)
  • For your References section:

    Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Cell Stem Cell. 2009 Jul 2. 5(1):97-110. 10.1016/j.stem.2009.05.023 PubMed 19540188