|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37800||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerKeith Joung lab @ MGH/HMS
- Backbone size w/o insert (bp) 5709
- Total vector size (bp) 5973
Modifications to backboneThe backbone was digested using XbaI/BamHI to insert the zinc finger sequence.
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namePIGA zinc finger R2
Insert Size (bp)264
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe insert and backbone were from Dr. Keith Joung's lab in Massachusetts General Hospital/Harvard Medical School.
Terms and Licenses
Please also cite the original article from Joung lab describing the construction of zinc finger nuclease:
Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, et al. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell. 2008;31:294–301.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:PIGAZFN-R2 was a gift from Linzhao Cheng (Addgene plasmid # 37800 ; http://n2t.net/addgene:37800 ; RRID:Addgene_37800)
For your References section:Gene targeting of a disease-related gene in human induced pluripotent stem and embryonic stem cells. Zou J, Maeder ML, Mali P, Pruett-Miller SM, Thibodeau-Beganny S, Chou BK, Chen G, Ye Z, Park IH, Daley GQ, Porteus MH, Joung JK, Cheng L. Cell Stem Cell. 2009 Jul 2. 5(1):97-110. 10.1016/j.stem.2009.05.023 PubMed 19540188