pSG5-HA-ERR3 S45A
(Plasmid
#37849)
-
Depositing Lab
-
Depositing OrganizationGeorgetown University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 37849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 37849-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 37849-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepSG5
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5516
-
Modifications to backboneModifications to Backbone: HA tag and modified multiple cloning site introduced. Original reference for pSG5-HA: PMID 10381882
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)XL10 Gold
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameEstrogen-related receptor gamma
-
Alt nameERRg, ERR3
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)1416
-
Mutationchanged Serine 45 to Alanine (AGC to GCC)
-
Entrez GeneEsrrg (a.k.a. ER, ERR3, Errg, NR3, NR3B3, mKIAA0832)
- Promoter SV40
-
Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer T7
- (Common Sequencing Primers)
Resource Information
-
A portion of this plasmid was derived from a plasmid made byThe original, wild type pSG5-HA-ERR3 construct was generated by Dr. Michael Stallcup. Original reference: PMID 10428842
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 37849-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 37849-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSG5-HA-ERR3 S45A was a gift from Rebecca Riggins (Addgene plasmid # 37849 ; http://n2t.net/addgene:37849 ; RRID:Addgene_37849) -
For your References section:
ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer. Heckler MM, Thakor H, Schafer CC, Riggins RB. FEBS J. 2014 Mar 29. doi: 10.1111/febs.12797. 10.1111/febs.12797 PubMed 24684682
