Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||37850||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5516
Modifications to backboneModifications to Backbone: HA tag and modified multiple cloning site introduced. Original reference for pSG5-HA: PMID 10381882
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL10 Gold
Gene/Insert nameEstrogen-related receptor gamma
Alt nameERRg, ERR3
SpeciesM. musculus (mouse)
Insert Size (bp)1416
Mutationchanged Serines 57, 81, and 219 to Alanine (AGC to GCC for Ser57 and Ser219; TCG to GCG for Ser81)
Entrez GeneEsrrg (a.k.a. ER, ERR3, Errg, NR3, NR3B3, mKIAA0832)
- Promoter SV40
/ Fusion Protein
- HA (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site BglII (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe original, wild type pSG5-HA-ERR3 construct was generated by Dr. Michael Stallcup. Original reference: PMID 10428842
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pSG5-HA-ERR3 S57,81,219A was a gift from Rebecca Riggins (Addgene plasmid # 37850 ; http://n2t.net/addgene:37850 ; RRID:Addgene_37850)
For your References section:ERK/MAPK regulates ERRgamma expression, transcriptional activity, and receptor-mediated Tamoxifen resistance in ER+ breast cancer. Heckler MM, Thakor H, Schafer CC, Riggins RB. FEBS J. 2014 Mar 29. doi: 10.1111/febs.12797. 10.1111/febs.12797 PubMed 24684682