pCLC-mEos2
(Plasmid #38010)

• Depositing Lab: Xiaowei Zhuang
• Depositing Organization: Harvard University
  Located in United States of America
• Publication: Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8.

Backbone

• Vector backbone: pEYFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 5340
• Modifications to backbone: EYFP sequence removed with AgeI (5') and NotI (3'). Replaced with mEos2.
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Clathrin, light polypeptide (LCa)
• Alt name: Clta
• Species: M. musculus (mouse)
• Insert Size (bp): 657
• GenBank ID: NM_001080385
• Entrez Gene: Clta (a.k.a. Lca)
• Promoter: CMV
• Tag / Fusion Protein:
  • mEos2 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: AgeI (not destroyed)
• 5′ sequencing primer: CMV promoter

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mEos2 was originally a kind gift of Dr. Michael W. Davidson (Florida State University) and Dr. Loren L. Looger (Howards Hughes Medical Institute, Janelia Farm). Clathrin light chain was a kind of gift of Dr. James H. Keen (Kimmel Cancer Center).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.

DNA (Catalog # 38010-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 38010-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.