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pCLC-mEos2
(Plasmid #38010)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38010 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEYFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5340
  • Modifications to backbone
    EYFP sequence removed with AgeI (5') and NotI (3'). Replaced with mEos2.
  • Vector type
    Mammalian Expression, Bacterial Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Clathrin, light polypeptide (LCa)
  • Alt name
    Clta
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    657
  • GenBank ID
    NM_001080385
  • Entrez Gene
    Clta (a.k.a. AV026556, Lca)
  • Promoter CMV
  • Tag / Fusion Protein
    • mEos2 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site AgeI (not destroyed)
  • 5′ sequencing primer CMV promoter
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    mEos2 was originally a kind gift of Dr. Michael W. Davidson (Florida State University) and Dr. Loren L. Looger (Howards Hughes Medical Institute, Janelia Farm). Clathrin light chain was a kind of gift of Dr. James H. Keen (Kimmel Cancer Center).
  • Terms and Licenses

Depositor Comments

Note: The clathrin light chain has an A9D amino acid residue substitution. This is a natural variant that does not affect protein function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLC-mEos2 was a gift from Xiaowei Zhuang (Addgene plasmid # 38010 ; http://n2t.net/addgene:38010 ; RRID:Addgene_38010)
  • For your References section:

    Fast, three-dimensional super-resolution imaging of live cells. Jones SA, Shim SH, He J, Zhuang X. Nat Methods. 2011 Jun;8(6):499-508. Epub 2011 May 8. 10.1038/nmeth.1605 PubMed 21552254