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pFRT/TO/HIS/FLAG/HA-MYEF2
(Plasmid #38069)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38069 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFRT/TO/HIS/FLAG/HA-DEST
  • Backbone size w/o insert (bp) 5508
  • Total vector size (bp) 7311
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MYEF2 myelin expression factor 2
  • Alt name
    MEF-2; MST156; MSTP156; HsT18564
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1803
  • GenBank ID
    NM_016132.3
  • Entrez Gene
    MYEF2 (a.k.a. HsT18564, MEF-2, MST156, MSTP156, myEF-2)
  • Tag / Fusion Protein
    • HIS/FLAG/HA (N terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

Digest and NGS results suggest that the original Gateway entry vector is still present in the DNA stock from which the bacterial stocks are created. Because the entry vector does not contain a promoter for the insert or selection marker, it should not interfere with function.

To attempt to remove the entry vector, recipient scientists could try to cure the plasmid from isolated colonies. Alternatively, they can linearize the entry vector with BmtI, HpaI or NheI digest, which will not cut the expression vector, and retransform into bacteria.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT/TO/HIS/FLAG/HA-MYEF2 was a gift from Markus Landthaler (Addgene plasmid # 38069 ; http://n2t.net/addgene:38069 ; RRID:Addgene_38069)
  • For your References section:

    The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M. Mol Cell. 2012 Jun 8;46(5):674-90. 10.1016/j.molcel.2012.05.021 PubMed 22681889