|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38069||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5508
- Total vector size (bp) 7311
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameMYEF2 myelin expression factor 2
Alt nameMEF-2; MST156; MSTP156; HsT18564
SpeciesH. sapiens (human)
Insert Size (bp)1803
Entrez GeneMYEF2 (a.k.a. HsT18564, MEF-2, MST156, MSTP156, myEF-2)
/ Fusion Protein
- HIS/FLAG/HA (N terminal on backbone)
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer BGH (Common Sequencing Primers)
Digest and NGS results suggest that the original Gateway entry vector is still present in the DNA stock from which the bacterial stocks are created. Because the entry vector does not contain a promoter for the insert or selection marker, it should not interfere with function.
To attempt to remove the entry vector, recipient scientists could try to cure the plasmid from isolated colonies. Alternatively, they can linearize the entry vector with BmtI, HpaI or NheI digest, which will not cut the expression vector, and retransform into bacteria.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFRT/TO/HIS/FLAG/HA-MYEF2 was a gift from Markus Landthaler (Addgene plasmid # 38069 ; http://n2t.net/addgene:38069 ; RRID:Addgene_38069)
For your References section:The mRNA-Bound Proteome and Its Global Occupancy Profile on Protein-Coding Transcripts. Baltz AG, Munschauer M, Schwanhausser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M. Mol Cell. 2012 Jun 8;46(5):674-90. 10.1016/j.molcel.2012.05.021 PubMed 22681889