pBad-Xis/Int Reset Flipper
(Plasmid #38207)

• Depositing Lab: Drew Endy
• Publication: Bonnet et al Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21.

Backbone

• Vector backbone: J64100
• Backbone size w/o insert (bp): 2800
• Vector type: Synthetic Biology ; BioBrick cloning vector

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Bxb1-Excisionase
• Promoter: pBAD

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: pBAD-F

Gene/Insert 2

• Gene/Insert name: Bxb1 Integrase

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate
and increase the excisionase to integrase ratio. This construct was cloned in a plasmid
with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).