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pBad-Xis/Int Reset Flipper
(Plasmid #38207)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38207 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    J64100
  • Backbone size w/o insert (bp) 2800
  • Vector type
    Synthetic Biology ; BioBrick cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Bxb1-Excisionase
  • Promoter pBAD

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Bxb1 Integrase

Resource Information

  • Terms and Licenses

Depositor Comments

In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate
and increase the excisionase to integrase ratio. This construct was cloned in a plasmid
with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBad-Xis/Int Reset Flipper was a gift from Drew Endy (Addgene plasmid # 38207 ; http://n2t.net/addgene:38207 ; RRID:Addgene_38207)
  • For your References section:

    Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351