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pBad-Int Set Flipper
(Plasmid #38209)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 38209 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Bxb1 Integrase
  • Promoter pBAD/AraC
  • Tag / Fusion Protein
    • His (N terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

The Bxb1 integrase was cloned downstream of the PBAD/AraC promoter (BBa_I0500) on pSB3K1 plasmid bearing a p15A origin of replication (15-20 copies). This version of the integrase has a 6-His-tag which we found to stabilize the protein. Therefore, a weak RBS (BBa_B0031) and a LAA ssrA tag was added to reduce the basal expression of the enzyme.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBad-Int Set Flipper was a gift from Drew Endy (Addgene plasmid # 38209 ; http://n2t.net/addgene:38209 ; RRID:Addgene_38209)
  • For your References section:

    Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351