pBad-Int Set Flipper
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||38209||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeSynthetic Biology ; BioBrick cloning vector
Growth in Bacteria
Gene/Insert nameBxb1 Integrase
- Promoter pBAD/AraC
/ Fusion Protein
- His (N terminal on insert)
- Cloning method Unknown
- 5′ sequencing primer pBAD-F (Common Sequencing Primers)
Terms and Licenses
The Bxb1 integrase was cloned downstream of the PBAD/AraC promoter (BBa_I0500) on pSB3K1 plasmid bearing a p15A origin of replication (15-20 copies). This version of the integrase has a 6-His-tag which we found to stabilize the protein. Therefore, a weak RBS (BBa_B0031) and a LAA ssrA tag was added to reduce the basal expression of the enzyme.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pBad-Int Set Flipper was a gift from Drew Endy (Addgene plasmid # 38209 ; http://n2t.net/addgene:38209 ; RRID:Addgene_38209)
For your References section:Rewritable digital data storage in live cells via engineered control of recombination directionality. Bonnet J, Subsoontorn P, Endy D. Proc Natl Acad Sci U S A. 2012 Jun 5;109(23):8884-9. Epub 2012 May 21. 10.1073/pnas.1202344109 PubMed 22615351