pKM263-ScPPX
(Plasmid
#38327)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 38327 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepKM263
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Backbone manufacturerK. Melcher
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePPX1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GenePPX1 (a.k.a. YHR201C)
- Promoter T7
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- GST (N terminal on backbone)
- TEV (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pKM263-ScPPX was a gift from Florian Freimoser (Addgene plasmid # 38327 ; http://n2t.net/addgene:38327 ; RRID:Addgene_38327) -
For your References section:
Novel method for the quantification of inorganic polyphosphate (iPoP) in Saccharomyces cerevisiae shows dependence of iPoP content on the growth phase. Werner TP, Amrhein N, Freimoser FM. Arch Microbiol. 2005 Nov;184(2):129-36. Epub 2005 Oct 21. 10.1007/s00203-005-0031-2 PubMed 16184370
