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YCplac111-TOR1 (pAN42)
(Plasmid #39208)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39208 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    YCplac111
  • Backbone size w/o insert (bp) 6100
  • Vector type
    Yeast Expression
  • Selectable markers
    LEU2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TOR1
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    10000
  • Entrez Gene
    TOR1 (a.k.a. YJR066W, DRR1)
  • Promoter TOR1
  • Tag / Fusion Protein
    • myc (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Expresses myc-tagged TOR1 from the TOR1 promoter (CEN LEU2). The two myc-tags are integrated between codons 86 and 87 of TOR1.

The 14 kb SacI fragment from pPW20 (Helliwell et al., 1994) was cloned into YCplac111, and the sequence ATC CCC GGG TTA ATT AAC GGT GAA CAA AAG CTA ATC TCC GAG GAA GAC TTG AAC GGT GAA CAA AAA TTA ATC TCA GAA GAA GAC TTG AAC GGA CTC GAC GGT GAA CAA encoding two myc tags was integrated between codons 86 and 87 of TOR1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    YCplac111-TOR1 (pAN42) was a gift from Michael Hall (Addgene plasmid # 39208)

  • For your References section:

    Two TOR complexes, only one of which is rapamycin sensitive, have distinct roles in cell growth control. Loewith R, Jacinto E, Wullschleger S, Lorberg A, Crespo JL, Bonenfant D, Oppliger W, Jenoe P, Hall MN. Mol Cell. 2002 Sep;10(3):457-68. 10.1016/S1097-2765(02)00636-6 PubMed 12408816
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