pCI-HA-mAVO3 (pRL62-3)
(Plasmid #39210)

• Depositing Lab: Michael Hall
• Publication: Jacinto et al Nat Cell Biol. 2004 Nov;6(11):1122-8. Epub 2004 Oct 3.

Backbone

• Vector backbone: pCI-HA
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4000
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mAVO3
• Alt name: Mammalian AVO3
• Species: M. musculus (mouse)
• Mutation: G5S and I15V
• Entrez Gene: Rictor (a.k.a. 4921505C17Rik, 6030405M08Rik, AVO3, D530039E11Rik)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: MluI/BssHII (destroyed during cloning)
• 3′ cloning site: SmaI (not destroyed)
• 5′ sequencing primer: CMV-F

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pCI-HA-based construct (with MluI/BssHII-SmaI sites) that expresses M. musculus mAVO3 N-terminally fused to an HA-epitope.

Addgene's sequencing results found G5S and I15V mutations in the AVO3 sequence when compared to GenBank ID NP_084444.3. This plasmid functions as described in the associated publication.