pMYOCWT-EGFP
(Plasmid #39326)

• Depositing Lab: Beatrice Yue
• Publication: Koga et al Am J Pathol. 2010 Jan;176(1):343-52. Epub 2009 Dec 3.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4733
• Total vector size (bp): 6221
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myocilin wild-type
• Species: H. sapiens (human)
• Insert Size (bp): 1527
• Entrez Gene: MYOC (a.k.a. GLC1A, GPOA, JOAG, JOAG1, TIGR)
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CMV promoter forward
• 3′ sequencing primer: EGFP reverse

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry