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pDEST-N112-MBP
(Plasmid #39342)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 39342 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pDEST-N112
  • Backbone manufacturer
    Addgene plasmid #39340
  • Backbone size (bp) 7820
  • Modifications to backbone
    MBP was inserted into the DraIII site of pDEST-N112 such that the 5' DraIII site is maintained, but destroying the 3' one.
  • Vector type
    Bacterial Expression
  • Promoter T7, lacO
  • Tags / Fusion Proteins
    • 10xHis (N terminal on backbone)
    • MBP (N terminal on backbone)
    • V5 (C terminal on backbone)
    • 6xHis (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer T7 promoter
  • 3′ sequencing primer T7 terminal primer
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-N112-MBP was a gift from John McCafferty (Addgene plasmid # 39342)

  • For your References section:

    Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression. Dyson MR, Shadbolt SP, Vincent KJ, Perera RL, McCafferty J. BMC Biotechnol. 2004 Dec 14;4:32. 10.1186/1472-6750-4-32 PubMed 15598350
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