|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||39530||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
SpeciesM. musculus (mouse)
Entrez GeneAkt1 (a.k.a. Ak, Akt, LTR-akt, PK, PKB, PKB/A, PKB/Akt, PKBalpha, Rac)
/ Fusion Protein
- ER (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site SalI/XhoI (destroyed during cloning)
- 5′ sequencing primer CMV fwd
- 3′ sequencing primer BGH_rev_primer (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
mAkt-HA-ER was cloned from pWZLneo-mAkt-HA-ER from kohn et al(1998) JBC 273(19)11937-11943.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3-mAkt-ER was a gift from Julian Downward (Addgene plasmid # 39530 ; http://n2t.net/addgene:39530 ; RRID:Addgene_39530)
For your References section:Akt/PKB localisation and 3' phosphoinositide generation at sites of epithelial cell-matrix and cell-cell interaction. Watton SJ, Downward J. Curr Biol. 1999 Apr 22;9(8):433-6. 10.1016/S0960-9822(99)80192-4 PubMed 10226029