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pCA-G-intron-G
(Plasmid #40027)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40027 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Total vector size (bp) 5876
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP exon 1
  • Alt name
    mut4EGFP
  • Mutation
    nucleotides 1-274
  • Promoter CAG (chicken beta globin promoter and CMV enhancer)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    GFP exon 2
  • Alt name
    mut4EGFP
  • Mutation
    nucleotides 275-stop
  • Promoter CAG (chicken beta globin promoter and CMV enhancer)

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    beta-globin intron
  • Mutation
    insertion of a loxP site
  • Promoter CAG (chicken beta globin promoter and CMV enhancer)

Cloning Information for Gene/Insert 3

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCA-G-intron-G was a gift from Liqun Luo (Addgene plasmid # 40027 ; http://n2t.net/addgene:40027 ; RRID:Addgene_40027)
  • For your References section:

    Extensions of MADM (Mosaic Analysis with Double Markers) in Mice. Tasic B, Miyamichi K, Hippenmeyer S, Dani VS, Zeng H, Joo W, Zong H, Chen-Tsai Y, Luo L. PLoS One. 2012;7(3):e33332. Epub 2012 Mar 27. 10.1371/journal.pone.0033332 PubMed 22479386