Skip to main content
Addgene
Open Menu

pAM2195
(Plasmid #40245)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40245 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart
DNA 40245-D50 50 μg of DNA in Tris buffer $495
Add to Cart
DNA 40245-D100 100 μg of DNA in Tris buffer $585
Add to Cart

Backbone

  • Vector backbone
    pAM1582
  • Total vector size (bp) 16600
  • Vector type
    Cyanobacteria cloning vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PpsbAI::luxCDE, PpsbAI::luxAB
  • Species
    Synechococcus elongatus PCC 7942
  • Insert Size (bp)
    5400
  • Promoter n/a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site n/a (unknown if destroyed)
  • 3′ cloning site n/a (unknown if destroyed)
  • 5′ sequencing primer n/a
  • 3′ sequencing primer n/a
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NSII.1 vector

Lux Genes: luxAB & luxCDE

synthesizes luciferase and substrate in S. elongatus

SmaI--PvuII fragment containing PpsbAI::luxCDE was cloned into SmaI site of pAM1582. Orientation of PpsbAI::luxCDE is same as that of PpsbAI::luxAB. NS2.1, PpsbAI::luxCDE, PpsbAI::luxAB plasmid.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAM2195 was a gift from Susan Golden (Addgene plasmid # 40245 ; http://n2t.net/addgene:40245 ; RRID:Addgene_40245)

  • For your References section:

    Detection of rhythmic bioluminescence from luciferase reporters in cyanobacteria. Mackey SR, Ditty JL, Clerico EM, Golden SS. Methods Mol Biol. 2007;362:115-29. 10.1007/978-1-59745-257-1_8 PubMed 17417005
Related items:
Commonly requested with: