Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||40269||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)XL10 Gold
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)3765
Entrez GeneERBB2 (a.k.a. CD340, HER-2, HER-2/neu, HER2, MLN 19, MLN-19, NEU, NGL, TKR1, VSCN2, c-ERB-2, c-ERB2, p185(erbB2))
- Promoter CMV
/ Fusion Protein
- ecfp (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site nheI (not destroyed)
- 3′ cloning site AgeI (not destroyed)
- 5′ sequencing primer CMV (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequence shows that the mutation is actually K753M. The discrepancy in the numbering is due to the presence of a cleaved signal peptide making the mature protein shorter.
There is also an I655V polymorphism in the sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:perbB2K732M-ECFP was a gift from Martin Offterdinger (Addgene plasmid # 40269 ; http://n2t.net/addgene:40269 ; RRID:Addgene_40269)
For your References section:Prolonged EGFR signaling by ERBB2-mediated sequestration at the plasma membrane. Offterdinger M, Bastiaens PI. Traffic. 2008 Jan;9(1):147-55. Epub 2007 Nov 13. 10.1111/j.1600-0854.2007.00665.x PubMed 17956594
Map uploaded by the depositor.