pUAST-NachBac-EGFP
(Plasmid
#40282)
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Depositing Lab
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Depositing OrganizationNational Institute of Mental Health (NIMH)
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 40282 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 40282-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 40282-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUAST
- Backbone size w/o insert (bp) 8914
- Total vector size (bp) 10552
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Vector typeDrosophila
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNaChBac-EGFP
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SpeciesBacillus halodurans
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GenBank IDBA000004.3
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Tag
/ Fusion Protein
- EGFP (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 40282-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 40282-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUAST-NachBac-EGFP was a gift from Benjamin White (Addgene plasmid # 40282 ; http://n2t.net/addgene:40282 ; RRID:Addgene_40282) -
For your References section:
Functional dissection of a neuronal network required for cuticle tanning and wing expansion in Drosophila. Luan H, Lemon WC, Peabody NC, Pohl JB, Zelensky PK, Wang D, Nitabach MN, Holmes TC, White BH. J Neurosci. 2006 Jan 11;26(2):573-84. 10.1523/JNEUROSCI.3916-05.2006 PubMed 16407556