RSVpGL3 (RSV promoter-luciferase in pGL3-basic)
(Plasmid #40343)

• Depositing Lab: Alexander Dent
• Publication: Vasanwala et al J Immunol. 2002 Aug 15;169(4):1922-9.

Backbone

• Vector backbone: pGL3-basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4818
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RSV promoter
• Alt name: Rous sarcoma virus (RSV) promoter
• Species: Rous sarcoma virus (RSV)
• Promoter: RSV
• Tag / Fusion Protein:
  • Luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: RVprimer3
• 3′ sequencing primer: LucNrev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The Rous sarcoma virus (RSV) promoter reporter was constructed by isolating the RSV promoter from pRc/RSV (Invitrogen) via BglII and HindIII sites and cloned into pGL3-basic by the same sites.