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Addgene

BCL6-H2Kk-RV (H2K-BCL6)
(Plasmid #40352)

Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 40352 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    MSCV2.1-H2Kk
  • Backbone manufacturer
    Mark Kaplan
  • Modifications to backbone
    Mouse H2K-k cDNA cloned after MSCV2.1 IRES
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BCL6
  • Alt name
    BCL-6
  • Alt name
    B-cell CLL/lymphoma 6
  • Species
    H. sapiens (human)
  • GenBank ID
    NG_007149.1
  • Entrez Gene
    BCL6 (a.k.a. BCL5, BCL6A, LAZ3, ZBTB27, ZNF51)
  • Tag / Fusion Protein
    • IRES mouse H2Kk (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (destroyed during cloning)
  • 3′ cloning site BamHI/BglII (destroyed during cloning)
  • 5′ sequencing primer pLXSN_5
  • 3′ sequencing primer IRES-R2 (gacggcaatatggtggaaa)
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

A MSCV2.1 retroviral vector was modified by cloning of mouse H2K-k cDNA after the IRES sequence to make MSCV2.1-H2Kk. MSCV2.1-H2Kk was obtained from Dr. Mark Kaplan. MSCV2.1-H2Kk was then cut with BglII and a human BCL6 cDNA with BamHI ends was cloned into the BglII site.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    BCL6-H2Kk-RV (H2K-BCL6) was a gift from Alexander Dent (Addgene plasmid # 40352 ; http://n2t.net/addgene:40352 ; RRID:Addgene_40352)
  • For your References section:

    Transcriptional repressor BCL6 controls Th17 responses by controlling gene expression in both T cells and macrophages. Mondal A, Sawant D, Dent AL. J Immunol. 2010 Apr 15;184(8):4123-32. Epub 2010 Mar 8. 10.4049/jimmunol.0901242 PubMed 20212093