pRMH120
(Plasmid #40593)

• Depositing Lab: Chandra Tucker
• Publication: Hughes et al Methods. 2012 Aug 15.

Backbone

• Vector backbone: p414TEF
  (Search Vector Database)
• Backbone size w/o insert (bp): 5414
• Total vector size (bp): 10860
• Modifications to backbone: Gal4BD-CRY2PHR inserted at TEF MCS, pADH-Gal4AD-CIBFL-tADH inserted at KpnI site
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: CRY2
• Alt name: cryptochrome 2
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1500

Gene/Insert 2

• Gene/Insert name: CIB1
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 1000

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry