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EEA1-mRFP
(Plasmid #40733)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 40733 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    N/A
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EEA1
  • Alt name
    Early Endosome Antigen1
  • Species
    H. sapiens (human)
  • Entrez Gene
    EEA1 (a.k.a. MST105, MSTP105, ZFYVE2)
  • Tag / Fusion Protein
    • mRFP (N terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Tom Kirchhausen

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    EEA1-mRFP was a gift from Tom Kirchhausen (Addgene plasmid # 40733)

  • For your References section:

    Perforin pores in the endosomal membrane trigger the release of endocytosed granzyme B into the cytosol of target cells. Thiery J, Keefe D, Boulant S, Boucrot E, Walch M, Martinvalet D, Goping IS, Bleackley RC, Kirchhausen T, Lieberman J. Nat Immunol. 2011 Jun 19;12(8):770-7. doi: 10.1038/ni.2050. 10.1038/ni.2050 PubMed 21685908
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