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pcDNA3-eGFP-LIC
(Plasmid #40768)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 40768 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone size (bp) 6000
  • Modifications to backbone
    Parent vector is addgene #13031. N-terminal eGFP with ligation independent cloning site compatible with primers made for pMCSGx vectors (Midwest Center for Structural Genomics)
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)
  • Tag / Fusion Protein
    • eGFP (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL1 Blue
  • Copy number
    High Copy

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Parent is Addgene #13031
  • Article Citing this Plasmid

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-eGFP-LIC was a gift from Dustin Maly (Addgene plasmid # 40768 ; http://n2t.net/addgene:40768 ; RRID:Addgene_40768)
  • For your References section:

    Conformation-Selective ATP-Competitive Inhibitors Control Regulatory Interactions and Noncatalytic Functions of Mitogen-Activated Protein Kinases. Hari SB, Merritt EA, Maly DJ. Chem Biol. 2014 May 22;21(5):628-35. doi: 10.1016/j.chembiol.2014.02.016. Epub 2014 Apr 3. 10.1016/j.chembiol.2014.02.016 PubMed 24704509