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pcDNA3.2/DEST/hCx43-E2HA-stop
(Plasmid #40909)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 40909 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.2/v5-DEST
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 7711
  • Total vector size (bp) 8893
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Connexin 43 internal HA tag
  • Alt name
    GJA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • Mutation
    HA tag inserted in second extracellular loop
  • GenBank ID
    NM_000165.3
  • Entrez Gene
    GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
  • Promoter CMV

Cloning Information

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.2/DEST/hCx43-E2HA-stop was a gift from Robin Shaw (Addgene plasmid # 40909 ; http://n2t.net/addgene:40909 ; RRID:Addgene_40909)
  • For your References section:

    Actin cytoskeleton rest stops regulate anterograde traffic of connexin 43 vesicles to the plasma membrane. Smyth JW, Vogan JM, Buch PJ, Zhang SS, Fong TS, Hong TT, Shaw RM. Circ Res. 2012 Mar 30;110(7):978-89. Epub 2012 Feb 9. 10.1161/CIRCRESAHA.111.257964 PubMed 22328533