pRS414-GPD-6xHis-Hsp82
(Plasmid #41188)

• Depositing Lab: Dan Bolon
• Publication: Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-95. Epub 2007 Oct 1.

Backbone

• Vector backbone: pRS414
• Backbone size w/o insert (bp): 5700
• Total vector size (bp): 7800
• Modifications to backbone: multiple cloning site altered to remove some restriction sites.
• Vector type: Yeast Expression
• Selectable markers: TRP1

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: S. cerevisiae Hsp82
• Alt name: Hsp90
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 2130
• Promoter: GPD
• Tag / Fusion Protein:
  • 6xHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: GPD forward
• 3′ sequencing primer: CYC reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry