|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||42149||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2172
- Total vector size (bp) 2994
Vector typeWorm Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesC. elegans (nematode), Synthetic
Insert Size (bp)822
Terms and Licenses
Nb. CDS terminates with two in-frame stop codons [TAA-TAG]
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pNH030 was a gift from Colin Dolphin (Addgene plasmid # 42149 ; http://n2t.net/addgene:42149 ; RRID:Addgene_42149)
For your References section:A simplified counter-selection recombineering protocol for creating fluorescent protein reporter constructs directly from C. elegans fosmid genomic clones. Hirani N, Westenberg M, Gami MS, Davis P, Hope IA, Dolphin CT. BMC Biotechnol. 2013 Jan 3;13(1):1. 10.1186/1472-6750-13-1 PubMed 23281894