pmAmetrine1.2
(Plasmid #42171)

• Depositing Lab: Robert Campbell
• Publication: Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. doi: 10.1021/ac202595g. Epub 2011 Nov 28.

Backbone

• Vector backbone: pBAD-His B
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 4100
• Total vector size (bp): 4800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mAmetrine1.2
• Species: Synthetic
• Insert Size (bp): 720
• Promoter: araBAD
• Tag / Fusion Protein:
  • 6His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: GGTCCCCTCTCTACTGTTTCTCCAT
• 3′ sequencing primer: CCCAAGGCGTTTCACTTCTGAGTT

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mAmetrine1.2 in an intensively engineered variant of GFP.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry