|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||27798||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonemVenus N1
Backbone manufacturerAddgene plasmid# 27793
- Backbone size w/o insert (bp) 3984
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert namemAmber N1
Insert Size (bp)720
- Cloning method Restriction Enzyme
- 5′ cloning site NheI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer CCAAAATCAACGGGACTTTCC
- 3′ sequencing primer CAGGTTCAGGGGGAGGTGTGG (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Amber was created by mutating Tyrosine 67 in Venus to Cysteine (Y67C).
See Addgene's sequencing results for detailed MCS and Amber. This plasmid is designed to clone a gene of interest upstream and in-frame to create a C-terminal Amber fusion protein.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Amber N1 was a gift from Steven Vogel (Addgene plasmid # 27798 ; http://n2t.net/addgene:27798 ; RRID:Addgene_27798)
For your References section:Cerulean, Venus, and VenusY67C FRET reference standards. Koushik SV, Chen H, Thaler C, Puhl HL, Vogel SS. Biophys J. 2006 Dec 15. 91(12):L99-L101. 10.1529/biophysj.106.096206 PubMed 17040988