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pCEFL EGFP-GABARAPL1
(Plasmid #42293)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 42293 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pCEFL EGFP
  • Backbone size w/o insert (bp) 6751
  • Total vector size (bp) 7105
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GABARAPL1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    354
  • Promoter Elongation factor 1
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII/BamHI (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP_5'
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    From IMAGE collection. Accession # BQ894446

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCEFL EGFP-GABARAPL1 was a gift from Mario Chiariello (Addgene plasmid # 42293)

  • For your References section:

    MAPK15/ERK8 stimulates autophagy by interacting with LC3 and GABARAP proteins. Colecchia D, Strambi A, Sanzone S, Iavarone C, Rossi M, Dall'armi C, Piccioni F, Verrotti di Pianella A, Chiariello M. Autophagy. 2012 Dec 1;8(12):1724-40. doi: 10.4161/auto.21857. Epub 2012 Sep 4. 10.4161/auto.21857 PubMed 22948227
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