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pF2-DTA
(Plasmid #42521)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42521 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pF2
  • Total vector size (bp) 3253
  • Vector type
    Mouse Targeting
  • Selectable markers
    mFabI

Growth in Bacteria

  • Bacterial Resistance(s)
    Triclosan
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Can be propagated in any E. coli strain. When grown on agar plates, 32 deg C incubation will yield larger colonies compared to 37 deg C grown ones. The colony size difference varies depending on the host strain. In broth culture, 37 deg C culture may produce higher yield of plasmid DNA. Use 1 micromolar triclosan for selection on plate and in broth cultures. Triclosan can be purchased from VWR (Cat. No. 80511-110. TRICLOSAN ANTIBIOTIC 1GM, Supplier: EMD Millipore, 647950-1GM). The triclosan powder is dissolved in pure DMSO in 50 millimolar and 1 millimolar (1000X) stocks and aliquoted, stored in -20.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    EF1a-DTA
  • Insert Size (bp)
    2581

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pF2-DTA was a gift from Terry Magnuson (Addgene plasmid # 42521 ; http://n2t.net/addgene:42521 ; RRID:Addgene_42521)
  • For your References section:

    A Novel Selection Marker for Efficient DNA Cloning and Recombineering in E. coli. Jang CW, Magnuson T. PLoS One. 2013;8(2):e57075. doi: 10.1371/journal.pone.0057075. Epub 2013 Feb 20. 10.1371/journal.pone.0057075 PubMed 23437314