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pDEST17-Tpt1
(Plasmid #42571)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42571 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST17
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Tpt1
  • Species
    S. cerevisiae (budding yeast)
  • GenBank ID
    CAA99116.1
  • Promoter T7 RNA Polmyerase
  • Tag / Fusion Protein
    • 6xHistidine (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See publication for expression protocol. Expressed in BL21 (DE3) RIPLE cells from Stratagene. Highly expressed and soluble.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST17-Tpt1 was a gift from Stan Fields & Jay Hesselberth (Addgene plasmid # 42571 ; http://n2t.net/addgene:42571 ; RRID:Addgene_42571)
  • For your References section:

    Capture and sequence analysis of RNAs with terminal 2',3'-cyclic phosphates. Schutz K, Hesselberth JR, Fields S. RNA. 2010 Mar;16(3):621-31. Epub 2010 Jan 14. 10.1261/rna.1934910 PubMed 20075163