pMCSG49-AlleyCat7
(Plasmid #42591)

• Depositing Lab: Ivan Korendovych
• Publication: Moroz et al Angew Chem Int Ed Engl. 2013 Apr 29. doi: 10.1002/anie.201302339.

Backbone

• Vector backbone: pMCSG49
• Backbone manufacturer: Midwest Center for Structural Genomics
• Backbone size w/o insert (bp): 4278
• Total vector size (bp): 4511
• Modifications to backbone: deletion of Asparagine (AAT) at the unique SspI site (AATATT) and insertion of AAGGGTGATCCGGC after stop codon of the gene
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: Room Temperature
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: mutated C-terminal portion of calmodulin gene (M76-K148)
• Species: G. gallus (chicken)
• Insert Size (bp): 222
• Mutation: changed Phenylalanine 92 to Glutamine acid, Isoleucine 85 to Leucine, Alanine 88 to Glutamine, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Methionine 124 to Leucine, Alanine 128 to Threonine, Methionine 144 to Arginine
• Promoter: T7
• Tags / Fusion Proteins:
  • TEV (N terminal on insert)
  • His (N terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CCTGTACTTCCAATCCATGAAAGATACAGATAGC
• 3′ sequencing primer: TTATCCACTTCCAATGCCGGATCACCCTTTCAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note that numbering for mutations starts with Ala (the second amino acid) immediately after the starting Met.