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p3XFLAG-TAK1-K63A
(Plasmid #43933)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 43933 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 43933-D50 50 μg of DNA in Tris buffer $495
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DNA 43933-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    p3xFLAG-CMV-14
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6190
  • Total vector size (bp) 8010
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Transforming growth factor-beta-activated kinase 1
  • Alt name
    TAK1
  • Alt name
    MAP3K7
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1817
  • Mutation
    K63A
  • GenBank ID
    NM_172688.3
  • Entrez Gene
    Map3k7 (a.k.a. B430101B05, Tak1)
  • Tag / Fusion Protein
    • 3XFLAG (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer CMV Forward
  • 3′ sequencing primer hGH-PA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p3XFLAG-TAK1-K63A was a gift from Bryant Darnay (Addgene plasmid # 43933 ; http://n2t.net/addgene:43933 ; RRID:Addgene_43933)

  • For your References section:

    TAK1-dependent signaling requires functional interaction with TAB2/TAB3. Besse A, Lamothe B, Campos AD, Webster WK, Maddineni U, Lin SC, Wu H, Darnay BG. J Biol Chem. 2007 Feb 9;282(6):3918-28. Epub 2006 Dec 8. 10.1074/jbc.M608867200 PubMed 17158449
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