pLX-V5-IKKe K38A-Blast
              
              
                (Plasmid
                
                #44119)
              
            
            
            
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              Depositing Lab
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          Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 44119 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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            Vector backbonepLEX304
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              Vector typeLentiviral
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                Selectable markersBlasticidin
Growth in Bacteria
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            Bacterial Resistance(s)Ampicillin, 100 μg/mL
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            Growth Temperature37°C
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            Growth Strain(s)Stbl3
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              Growth instructionsRecommended growth media: Terrific broth (TB)
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            Copy numberHigh Copy
Gene/Insert
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                Gene/Insert nameIKKe K38A
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                  Alt nameIKBKE K38A
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                  Alt nameIKKe
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                  Alt nameIKBKE
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                    SpeciesH. sapiens (human)
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                  MutationLysine 38 Alanine
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                        Entrez GeneIKBKE (a.k.a. IKK-E, IKK-i, IKKE, IKKI)
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        Tag
        / Fusion Protein
    - V5 (C terminal on insert)
 
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer WPRE-R (Common Sequencing Primers)
Terms and Licenses
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        Academic/Nonprofit Terms
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      Industry Terms- Not Available to Industry
 
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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              For your Materials & Methods section: pLX-V5-IKKe K38A-Blast was a gift from William Hahn (Addgene plasmid # 44119)
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                For your References section: IkappaB kinase epsilon phosphorylates TRAF2 to promote mammary epithelial cell transformation. Shen RR, Zhou AY, Kim E, Lim E, Habelhah H, Hahn WC. Mol Cell Biol. 2012 Dec;32(23):4756-68. doi: 10.1128/MCB.00468-12. Epub 2012 Sep 24. 10.1128/MCB.00468-12 PubMed 23007157