IF-GFP-ATRX
(Plasmid #45444)

• Purpose: ATRX expression vector
• Depositing Lab: Michael Dyer
• Depositing Organization: St. Jude Children's Research Hospital
  Located in United States of America
• Publication: Valle-Garcia et al Springerplus. 2014 May 2;3:222. doi: 10.1186/2193-1801-3-222. eCollection 2014.
• Citations: 6 Articles

Backbone

• Vector backbone: pEGFP C2
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 12000
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Growth instructions: As ATRX is prone to insertion by IS10, you should sequence exon 8 and the entire sequence on occasion but especially after culturing in E. coli. A good primer for sequencing is GCCAACATGAACTCCAGTTATG.
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: ATRX
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 7400
• Mutation: isoform 2, missing codon E124
• Entrez Gene: ATRX (a.k.a. JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX)
• Promoter: PCMV
• Tags / Fusion Proteins:
  • GFP (N terminal on backbone)
  • HA (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XbaI (not destroyed)
• 5′ sequencing primer: EGFP N sequencing primer
• 3′ sequencing primer: EGFP C sequencing primer

Resource Information

• A portion of this plasmid was derived from a plasmid made by: It was made from a combination of ATRX sequence cloned by David Picketts and a short sequence engineered by Genscript.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The EcoRI restriction pattern is different between the plasmid described in the paper and the plasmid available from Addgene. This difference does not effect the function of the plasmid.

DNA (Catalog # 45444-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 45444-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.