Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45746||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5238
- Total vector size (bp) 11699
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Alt nameDHQS homolog, O-methyltransferase, ATP-grasp, NRPS-like
Insert Size (bp)6461
- Promoter T7
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET29b-Ava3858-3855 was a gift from Emily Balskus & Christopher T Walsh (Addgene plasmid # 45746 ; http://n2t.net/addgene:45746 ; RRID:Addgene_45746)
For your References section:The genetic and molecular basis for sunscreen biosynthesis in cyanobacteria. Balskus EP, Walsh CT. Science. 2010 Sep 24;329(5999):1653-6. doi: 10.1126/science.1193637. Epub 2010 Sep 2. 10.1126/science.1193637 PubMed 20813918
Map uploaded by the depositor.