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pcDNA3 MDM2 1-400
(Plasmid #45754)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 45754 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5446
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    MDM2 1-400
  • Alt name
  • Alt name
    E3 ubiquitin protein ligase
  • Alt name
  • Species
    H. sapiens (human)
  • Mutation
    aa 1-400, lacks catalytic RING domain
  • GenBank ID
  • Entrez Gene
    MDM2 (a.k.a. ACTFS, HDMX, hdm2)
  • Promoter CMV

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3 MDM2 1-400 was a gift from Robert Lefkowitz (Addgene plasmid # 45754)
  • For your References section:

    Regulation of receptor fate by ubiquitination of activated beta 2-adrenergic receptor and beta-arrestin. Shenoy SK, McDonald PH, Kohout TA, Lefkowitz RJ. Science. 2001 Nov 9;294(5545):1307-13. Epub 2001 Oct 4. 10.1126/science.1063866 PubMed 11588219