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hα1Ga-pDsRed
(Plasmid #45811)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 45811 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 45811-D50 50 μg of DNA in Tris buffer $495
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DNA 45811-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pDsRed-Express-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 11662
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hα1Ga
  • Alt name
    CACNA1G
  • Alt name
    Cav3.1a
  • Alt name
    calcium channel, voltage-dependent, T type, alpha 1G subunit
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6962
  • GenBank ID
    AF190860
  • Entrez Gene
    CACNA1G (a.k.a. Ca(V)T.1, Cav3.1, NBR13, SCA42, SCA42ND)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Cav3.1 insert retains its STOP codon, so there is no expression of the Ds-Red. The 5'UTR is gone and contains a new SalI site. The 3' end of insert ends in the endogenous Kpn1 site.
Identification of Cav3.1 is described in Nature. 1998 Feb 26;391(6670):896-900.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    hα1Ga-pDsRed was a gift from Edward Perez-Reyes (Addgene plasmid # 45811 ; http://n2t.net/addgene:45811 ; RRID:Addgene_45811)

Ready-to-use antibodies

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