|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||45828||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLidstrom Lab (Addgene plasmid 45826)
- Backbone size (bp) 7965
Modifications to backbonecreated by inserting a 1.0 kb fragment containing xylE (from pCM75) into pCM62 (Addgene plasmid 45826)cut with AflIII and HindIII, followed by the insertion of a 0.5 kb XbaI-XmnI trrnB fragment from pMTL20T1T2 into the blunted EcoRI site.
Vector typelow-background broad-host-range promoter-probe vector using xylE as a reporter gene
- Promoter none
Growth in Bacteria
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13-F20 (Common Sequencing Primers)
XylE replaces the lac promoter in pCM62 (Addgene plasmid 45826) and is used as a reporter to test promoter activity. A transcriptional terminator was added to reduce background activity.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCM130 was a gift from Mary Lidstrom (Addgene plasmid # 45828 ; http://n2t.net/addgene:45828 ; RRID:Addgene_45828)
For your References section:Development of improved versatile broad-host-range vectors for use in methylotrophs and other Gram-negative bacteria. Marx CJ, Lidstrom ME. Microbiology. 2001 Aug;147(Pt 8):2065-75. 10.1099/00221287-147-8-2065 PubMed 11495985