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pET15b/Shigella flexneri IpaA
(Plasmid #46178)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46178 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-15b
  • Backbone manufacturer
    EMD
  • Backbone size w/o insert (bp) 5700
  • Total vector size (bp) 7607
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    IpaA
  • Species
    Shigella flexneri
  • Insert Size (bp)
    1902
  • Promoter T7
  • Tag / Fusion Protein
    • His (N terminal on backbone)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

IpaA cDNA was amplified from pEC14, supplied by M. Venketesan (Walter Reed Army Institute of Research, Washington, DC),
using appropriate primers to introduce an NdeI site before the initiating ATG and an XhoI site downstream of the stop codon. The
PCR product was transformed into TOPO pCRII (Invitrogen). The NdeI and XhoI fragment of IpaA cDNA was isolated from a pCRII clone containing the correct sequence and inserted into pET15b vector (Novogen) to generate pET15b/IpaA.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/Shigella flexneri IpaA was a gift from Susan Craig (Addgene plasmid # 46178 ; http://n2t.net/addgene:46178 ; RRID:Addgene_46178)
  • For your References section:

    Spatial distribution and functional significance of activated vinculin in living cells. Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW. J Cell Biol. 2005 May 9;169(3):459-70. 10.1083/jcb.200410100 PubMed 15883197