pET15b/Shigella flexneri IpaA
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||46178||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5700
- Total vector size (bp) 7607
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)1902
- Promoter T7
/ Fusion Protein
- His (N terminal on backbone)
- Cloning method TOPO Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7 terminal (Common Sequencing Primers)
Terms and Licenses
IpaA cDNA was amplified from pEC14, supplied by M. Venketesan (Walter Reed Army Institute of Research, Washington, DC),
using appropriate primers to introduce an NdeI site before the initiating ATG and an XhoI site downstream of the stop codon. The
PCR product was transformed into TOPO pCRII (Invitrogen). The NdeI and XhoI fragment of IpaA cDNA was isolated from a pCRII clone containing the correct sequence and inserted into pET15b vector (Novogen) to generate pET15b/IpaA.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET15b/Shigella flexneri IpaA was a gift from Susan Craig (Addgene plasmid # 46178 ; http://n2t.net/addgene:46178 ; RRID:Addgene_46178)
For your References section:Spatial distribution and functional significance of activated vinculin in living cells. Chen H, Cohen DM, Choudhury DM, Kioka N, Craig SW. J Cell Biol. 2005 May 9;169(3):459-70. 10.1083/jcb.200410100 PubMed 15883197