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pHAGE PGK-GFP-IRES-LUC-W
(Plasmid #46793)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 46793 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pHAGE
  • Backbone manufacturer
    Richard Mulligan
  • Backbone size w/o insert (bp) 4874
  • Total vector size (bp) 9272
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    PGK
  • Alt name
    PGK promoter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    465

Gene/Insert 2

  • Gene/Insert name
    eGFP
  • Insert Size (bp)
    720
  • Entrez Gene
    eGFP (a.k.a. pPRS3a_01)

Gene/Insert 3

  • Gene/Insert name
    IRES
  • Alt name
    Internal ribosomal entry site
  • Insert Size (bp)
    578

Gene/Insert 4

  • Gene/Insert name
    Luciferase
  • Insert Size (bp)
    1653

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHAGE PGK-GFP-IRES-LUC-W was a gift from Darrell Kotton (Addgene plasmid # 46793 ; http://n2t.net/addgene:46793 ; RRID:Addgene_46793)
  • For your References section:

    Amelioration of emphysema in mice through lentiviral transduction of long-lived pulmonary alveolar macrophages. Wilson AA, Murphy GJ, Hamakawa H, Kwok LW, Srinivasan S, Hovav AH, Mulligan RC, Amar S, Suki B, Kotton DN. J Clin Invest. 2010 Jan . 120(1):379-89. 10.1172/JCI36666 PubMed 20038801