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pECIA2
(Plasmid #47032)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47032 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMT/BiP/V5/His A
  • Backbone manufacturer
    Invitrogen
  • Backbone size (bp) 4349
  • Vector type
    Insect Expression ; Secreted expression in Drosophila Culture
  • Promoter Metallothionein (Copper-Inducible)
  • Tags / Fusion Proteins
    • HRV 3C Protease Cleavage site (C terminal on backbone)
    • Fc(human IgG1) (C terminal on backbone)
    • V5 (C terminal on backbone)
    • Hexahistidine (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

Resource Information

Depositor Comments

Inducible with 0.8 uM Copper Sulfate. Targets proteins to the extracellular millieu using the Drosophila melanogaster BiP signal peptide. Expresses in Drosophila melanogaster cell lines, such as Schneider 2 (S2) cells. Published in Ozkan et al, Cell 154(1): 228 (2013); PMID: 23827685; doi: 10.1016/j.cell.2013.06.006

After recombination, transform and grow in DH5alpha. The plasmid is no longer chloramphenicol resistant after recombination.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pECIA2 was a gift from Chris Garcia (Addgene plasmid # 47032 ; http://n2t.net/addgene:47032 ; RRID:Addgene_47032)
  • For your References section:

    An Extracellular Interactome of Immunoglobulin and LRR Proteins Reveals Receptor-Ligand Networks. Ozkan E, Carrillo RA, Eastman CL, Weiszmann R, Waghray D, Johnson KG, Zinn K, Celniker SE, Garcia KC. Cell. 2013 Jul 3;154(1):228-39. doi: 10.1016/j.cell.2013.06.006. 10.1016/j.cell.2013.06.006 PubMed 23827685