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Flag Intersectin Short (human)
(Plasmid #47392)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47392 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Intersectin short
  • Alt name
    intersectin-s
  • Alt name
    ITSN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITSN1 (a.k.a. ITSN, SH3D1A, SH3P17)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (destroyed during cloning)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Flag Intersectin Short (human) was a gift from Peter McPherson (Addgene plasmid # 47392 ; http://n2t.net/addgene:47392 ; RRID:Addgene_47392)
  • For your References section:

    Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP. Hussain NK, Jenna S, Glogauer M, Quinn CC, Wasiak S, Guipponi M, Antonarakis SE, Kay BK, Stossel TP, Lamarche-Vane N, McPherson PS. Nat Cell Biol. 2001 Oct;3(10):927-32. 10.1038/ncb1001-927 PubMed 11584276