Flag Intersectin Short (human)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47392||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4754
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberLow Copy
Gene/Insert nameIntersectin short
SpeciesH. sapiens (human)
Entrez GeneITSN1 (a.k.a. ITSN, SH3D1A, SH3P17)
- Promoter CMV
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site SmaI (destroyed during cloning)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Flag Intersectin Short (human) was a gift from Peter McPherson (Addgene plasmid # 47392 ; http://n2t.net/addgene:47392 ; RRID:Addgene_47392)
For your References section:Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP. Hussain NK, Jenna S, Glogauer M, Quinn CC, Wasiak S, Guipponi M, Antonarakis SE, Kay BK, Stossel TP, Lamarche-Vane N, McPherson PS. Nat Cell Biol. 2001 Oct;3(10):927-32. 10.1038/ncb1001-927 PubMed 11584276