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GFP-Intersectin Short
(Plasmid #47394)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47394 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Intersectin short
  • Alt name
    intersectin-s
  • Alt name
    ITSN1
  • Species
    H. sapiens (human)
  • Entrez Gene
    ITSN1 (a.k.a. ITSN, SH3D1A, SH3P17)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-Intersectin Short was a gift from Peter McPherson (Addgene plasmid # 47394 ; http://n2t.net/addgene:47394 ; RRID:Addgene_47394)
  • For your References section:

    Endocytic protein intersectin-l regulates actin assembly via Cdc42 and N-WASP. Hussain NK, Jenna S, Glogauer M, Quinn CC, Wasiak S, Guipponi M, Antonarakis SE, Kay BK, Stossel TP, Lamarche-Vane N, McPherson PS. Nat Cell Biol. 2001 Oct;3(10):927-32. 10.1038/ncb1001-927 PubMed 11584276