Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEN_TT MEK5-DD-mRFP1
(Plasmid #47547)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 47547 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEN_TTmiRc2
  • Backbone manufacturer
    Addgene #25752
  • Backbone size w/o insert (bp) 4422
  • Vector type
    Entry vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Gentamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MEK5-DD
  • Alt name
    MEK5
  • Alt name
    MAP2K5
  • Alt name
    MAPKK5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2025
  • Mutation
    S311D, T315D
  • Entrez Gene
    MAP2K5 (a.k.a. HsT17454, MAPKK5, MEK5, PRKMK5)
  • Tag / Fusion Protein
    • mRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site MfeI (not destroyed)
  • 5′ sequencing primer pCEP-fwd
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN_TT MEK5-DD-mRFP1 was a gift from Kevin Janes (Addgene plasmid # 47547 ; http://n2t.net/addgene:47547 ; RRID:Addgene_47547)
  • For your References section:

    An ERK-p38 subnetwork coordinates host cell apoptosis and necrosis during coxsackievirus B3 infection. Jensen KJ, Garmaroudi FS, Zhang J, Lin J, Boroomand S, Zhang M, Luo Z, Yang D, Luo H, McManus BM, Janes KA. Cell Host Microbe. 2013 Jan 16;13(1):67-76. doi: 10.1016/j.chom.2012.11.009. Epub 2013 Jan 16. 10.1016/j.chom.2012.11.009 PubMed 23332156