Purpose(Empty Backbone) Destination plasmid with mClover for Golden Gate reaction of TALE assembly
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||47874||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepCAG-T7-TALEN (Sangamo)
Modifications to backboneFokI domain was replaced by mClover
Vector typeMammalian Expression, TALEN
- Promoter CAG
/ Fusion Protein
- mClover (C terminal on backbone)
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTALYM3 was a gift from Maria-Elena Torres-Padilla (Addgene plasmid # 47874 ; http://n2t.net/addgene:47874 ; RRID:Addgene_47874)
For your References section:Live visualization of chromatin dynamics with fluorescent TALEs. Miyanari Y, Ziegler-Birling C, Torres-Padilla ME. Nat Struct Mol Biol. 2013 Oct 6. doi: 10.1038/nsmb.2680. 10.1038/nsmb.2680 PubMed 24096363