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pRK5-myc-Miro2 R425V
(Plasmid #47898)

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Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 47898 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 47898-D50 50 μg of DNA in Tris buffer $495
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DNA 47898-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pRK5-myc
  • Backbone manufacturer
    Dr. Alan Hall, MRC-LMCB
  • Modifications to backbone
    Myc inserted into EcoRI/PstI site (sites destroyed).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Miro2 R425V
  • Alt name
    RHOT2
  • Alt name
    ras homolog family member T2
  • Alt name
    Miro-2
  • Species
    H. sapiens (human)
  • Mutation
    R425V, constitutively active mutant
  • GenBank ID
    AJ517413
  • Entrez Gene
    RHOT2 (a.k.a. ARHT2, C16orf39, MIRO-2, MIRO2, RASL)
  • Promoter CMV
  • Tag / Fusion Protein
    • myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-myc-Miro2 R425V was a gift from Pontus Aspenström (Addgene plasmid # 47898 ; http://n2t.net/addgene:47898 ; RRID:Addgene_47898)

  • For your References section:

    The atypical Rho GTPases Miro-1 and Miro-2 have essential roles in mitochondrial trafficking. Fransson S, Ruusala A, Aspenstrom P. Biochem Biophys Res Commun. 2006 Jun 2;344(2):500-10. Epub 2006 Apr 3. 10.1016/j.bbrc.2006.03.163 PubMed 16630562
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