pENTR-hMeCP2
(Plasmid
#48077)
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PurposeGateway entry clone
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Depositing Lab
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Sequence Information
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Please contact us if you would like Addgene to sequence a portion of this plasmid before you request it.
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Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48077 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepDONR223
- Backbone size w/o insert (bp) 5005
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Vector typeGateway Cloning
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMethyl-CpG Binding Protein 2 (Rett Syndrome)
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Alt nameMeCP2
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SpeciesH. sapiens (human)
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Insert Size (bp)1461
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Entrez GeneMECP2 (a.k.a. AUTSX3, MRX16, MRX79, MRXS13, MRXSL, PPMX, RS, RTS, RTT)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTR-hMeCP2 was a gift from Huda Zoghbi (Addgene plasmid # 48077) -
For your References section:
An AT-hook domain in MeCP2 determines the clinical course of Rett syndrome and related disorders. Baker SA, Chen L, Wilkins AD, Yu P, Lichtarge O, Zoghbi HY. Cell. 2013 Feb 28;152(5):984-96. doi: 10.1016/j.cell.2013.01.038. 10.1016/j.cell.2013.01.038 PubMed 23452848