pNI100-P100-U058-sfGFP
(Plasmid
#48265)
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PurposesfGFP protein expressed by the P100 promoter (BIOFAB: apFAB95) and U058 ribosome binding site (BIOFAB: apFAB690).
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 48265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepJ251
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Backbone manufacturerDNA2.0
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Modifications to backbonepACYC low copy origin
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH10B
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameP100-U058-sfGFP
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNI100-P100-U058-sfGFP was a gift from Hans Roubos (Addgene plasmid # 48265) -
For your References section:
Using synthetic biological parts and microbioreactors to explore the protein expression characteristics of Escherichia coli. Gorochowski TE, van den Berg E, Kerkman R, Roubos JA, Bovenberg RA. ACS Synth Biol. 2014 Mar 21;3(3):129-39. doi: 10.1021/sb4001245. Epub 2013 Dec 10. 10.1021/sb4001245 PubMed 24299494